Macvector

Author: s | 2025-04-25

Happy Holidays from the MacVector Team; MacVector Pro is now MacVector Pro with Assembler; MacVector 18.7.3: a minor bug fix release; Navigation; Getting Started with MacVector. Getting Started with MacVector: An overview of primer design workflows in MacVector. Getting Started with MacVector: assembling sequence data with MacVector and

MacVector Pro is now MacVector Pro with Assembler

MacVector Installers and Updaters Which installer do I need? The latest version of MacVector that you can run is determined by the maintenance end date of your license. You can find this by running MacVector and choosing the MacVector | About MacVector menu item; Typically, the maintenance end date is set to 12 months after the last time that you or your institution last purchased MacVector, an upgrade, or renewed a yearly maintenance contract. If you believe the displayed date is incorrect, please check that you entered the most recent activation code sent from [email protected]. Next, look in the table below and find the most recent installer that your license can run; If the maintenance end date is more recent than this date Use this installer 1st June 2024 MacVector 18.7.1 1st July 2023 MacVector 18.6.4 1st November 2022 MacVector 18.5.1 1st August 2021 MacVector 18.2.5 1st February 2021 MacVector 18.1.5 1st December 2020 MacVector 18.0.1 1st February 2020 MacVector 17.5.4 1st November 2018 MacVector 17.0.10 1st September 2017 MacVector 16.0.10 1st February 2017 MacVector 15.5.3 1st August 2016 MacVector 15.1.5 1st June 2016 MacVector 15.0.2 1st November 2015 MacVector 14.5.3 1st February 2015 MacVector 14.0.6 1st September 2014 MacVector 13.5.5 1st November 2013 MacVector 13.0.7 1st October 2012 MacVector 12.7.5 1st May 2012 MacVector 12.6 1st October 2011 MacVector 12.5.1 1st December 2010 MacVector 12.0.6 1st December 2009 MacVector 11.1.2 1st July 2009 MacVector 11.0.4 1st March 2009 MacVector 10.6.0 1st October 2008 MacVector 10.5.3 1st January 2008 MacVector 10.0.2 1st April 2007 MacVector 9.5.4 Older Installers and Updaters These releases will only run on very old operating systems and hardware. They require the old purple USB hardware dongles to run. It is unlikely you will ever need to download and install these unless you have some very specific requirements and have discussed your needs with MacVector Support personnel. MacVector 9.0.2 MacVector 8.1 MacVector 7.2.3

MacVector, Inc. announces the release of MacVector 10.6.

That all we do keeps MacVector future proof and to be a modern macOS application that you can rely on.How to upgrade to MacVector 18.5If you have a maintenance contract that was active on 1st November, 2022, then you can install MacVector 18.5. You must be running macOS High Sierra to macOS Ventura. You will be prompted to automatically update within the next few days.You can also download the installer and do it manually now.If you have an older version of MacVector then download the trial and request an upgrade quote.Even if you have downloaded the trial in the past then downloading a new trial will give you a fresh 21 days to evaluate MacVector.When a trial license expires it becomes MacVector Free. So if you decide against upgrading then you can just delete the trial license and easily go back to your current version. It’s risk free as MacVector files are backwards compatible. MacVector 18.5 is macOS Ventura ready By Chris | Published: October 25, 2022 It’s that time of year again. Apple have just released macOS Ventura and we are very pleased to announce our current release, MacVector 18.2 has been tested on macOS Ventura and is compatible.Not only that but we have just a few weeks of testing left before our next release. MacVector 18.5 has been developed on macOS Ventura since the first developers beta build was available. MacVector 18.5 has been extensively tested on macOS Ventura too.Compatibility of previous versionsFor versions of MacVector before the current one you can check compatibility on a table which we update after every official release of macOS. For versions of MacVector released over the past few years it is likely that they will work fine. Our developers strive to future proof MacVector, and it is only when Apple make

Getting Started with MacVector: assembling sequence data with MacVector

Chris | Published: October 13, 2021 MacVector will directly import SnapGene DNA files. You just need to use FILE | OPEN or double click the file.This is very useful when downloading plasmid sequences from the wonderful Addgene plasmid repository.Here’s a plasmid sequence downloaded from the Addgene website in Snapgene format. It’s been opened directly in MacVector by double clicking the file. A Snapgene plasmid imported directly into MacVectorImport a Snapgene sequence into MacVectorThis can be as easy as just double clicking on a sequence and it will open directly in MacVector. However, if it opens in SnapGene instead then:Open MacVectorGo to FILE | OPEN…Select the SnapGene file and click OKWhen you import a Snapgene file the appearance will be very similar. The colors of features will be the same as the original. However, there are some aspects to the display that are not the same between the two applications. For example MacVector has multiple levels (up to six) outside and inside a plasmid and will always try to place features so that no feature overlaps another. However, Snapgene will always place features on the same two levels and so features sometimes overlap.This sequence was opened using the MacVector defaults. However, MacVector’s graphics are highly customizable and you can adjust the graphical settings to display the plasmid exactly as you want. For example you may prefer features to be displayed on just those two levels instead of being distributed over the multiple levels as per the default settings.MacVector will directly import many file formats such as common sequence formats such as Genbank, FASTA, FASTQ plus from software packages such as Sequencher Projects and Serial Cloner, This entry was posted in Uncategorized and tagged annotation, formats, Importing files, snapgene. Bookmark the permalink. Both comments and trackbacks are currently closed.. Happy Holidays from the MacVector Team; MacVector Pro is now MacVector Pro with Assembler; MacVector 18.7.3: a minor bug fix release; Navigation; Getting Started with MacVector. Getting Started with MacVector: An overview of primer design workflows in MacVector. Getting Started with MacVector: assembling sequence data with MacVector and Happy Holidays from the MacVector Team; MacVector Pro is now MacVector Pro with Assembler; MacVector 18.7.3: a minor bug fix release; Navigation; Getting Started with MacVector. Getting Started with MacVector: An overview of primer design workflows in MacVector. Getting Started with MacVector: assembling sequence data with MacVector and

Getting Started with MacVector: Getting your sequence into MacVector

Significant changes that older versions may stop working.Such significant changes include:MacVector 13.5 and all older versions will not run on macOS Catalina and earlier. This is due to Apple moving fully to a 64 bit operating system.MacVector 14.0 relies on an Apple library that Apple removed in MacOS Big Sur and later macOS releases. MacVector is macOS Ventura ready By Chris | Published: October 25, 2022 It’s that time of year again. Apple have just released macOS Ventura and we are very pleased to announce our current release, MacVector 18.2 is macOS Ventura ready.Not only that but we have just a few weeks of testing left before our next release. MacVector 18.5 has been developed on macOS Ventura since the first developers beta build was available. MacVector 18.5 has been extensively tested on macOS Ventura too.Compatibility of previous versionsFor versions of MacVector before the current one you can check compatibility on a table which we update after every official release of macOS. For versions of MacVector released over the past few years it is likely that they will work fine. Our developers strive to future proof MacVector, and it is only when Apple make significant changes that older versions may stop working.Such significant changes include:MacVector 13.5 and all older versions will not run on macOS Catalina and earlier. This is due to Apple moving fully to a 64 bit operating system.MacVector 14.0 relies on an Apple library that Apple removed in MacOS Big Sur and later macOS releases. MacVectorTip: Create custom Codon Usage Tables for ORF analysis and reverse translation By Chris | Published: October 19, 2022 MacVector uses codon usage tables (also called “Codon Bias”) to help you predict protein coding regions in the Analyze | Nucleic Acid Analysis Toolbox functions and also the protein Analyze | Reverse Translation...

MacVector 9.0.2 Installer

To align DNA sequences based on their amino acid translations and multiple protein sequences can now be aligned to a single reference protein sequence.Translated Multiple Sequence Alignments: Align DNA sequences based on their amino acid translations. Display DNA sequences and their translations at the same time. Align the protein sequences using ClustalW, Muscle or T-Coffee to see the effect on the underlying DNA sequences. Directly edit the DNA sequences and immediately see the impact of the change on the amino acid alignments.Align proteins against a reference: You can use a protein sequence as a reference so that the display keys off that sequence when showing similarities. This allows you to view proteins in a similar way to the DNA Align To Reference interface.Applescript and Auto Annotate: Auto-annotation has joined the growing number of MacVector tools that support Applescript. Batch annotate folders of blank sequences. Example scripts provided.Check out the release notes for full details of this release.How to update to MacVector 15If you have active maintenance and are running MacVector 13.0.1 or later then you should have been notified about the new release already. At that point you have the option to automatically upgrade to MacVector 15. To install this version, you must have a maintenance contract that was active on 1 June 2016. If you are running OS X 10.6.8, the semi-automatic updater is not supported and you should download the full updater direct.If you have an older version of MacVector then download the trial and request an upgrade quote.If have downloaded the trial in the past then downloading a new trial will give you a fresh 21 days to evaluate MacVector even if a previous trial license had expired.Remember that when a trial expires it becomes MacVector Free. Posted in Releases | Tagged releases

What’s New in MacVector.

Function. For example, here are the Plus and Minus strand Staden Codon Preference plots aligned under the Plus/Minus ORFs for a Streptomyces coelicolor cosmid fragment using the Streptomyces coelicolor.bias codon usage file.While MacVector includes a collection of common codon usage tables (Human, Mouse, Drosophila, E. coli etc.) in the /MacVector/Codon Bias Tables/ folder, there may be times when you need a custom table. We do have a utility that can convert data in the appropriate format into a MacVector .bias file, but unfortunately it will not yet run on recent macOS releases. However, we are happy to generate these files for you if you send us the raw data. To do this, go to and search for your preferred organism. You should ask to see the data in the CodonFrequency GCG format.Copy the text starting from AmAcid to the end of the data.Paste it into an email and send it to [email protected] and we will send you back a .bias file. macOS’s tabbed windows and MacVector By Chris | Published: September 23, 2022 One of the lesser known features of macOS is the ability to store all open documents of an application in tabs. Tabs were initially introduced for the Finder, but macOS Mavericks saw them apply to supported application document windows too. MacVector has supported tabs since their introduction, however, by default the Tab Bar is turned off.To view the Tab Bar in MacVector then use:VIEW | SHOW/HIDE TAB BARHowever, to control the behaviour when you open new documents you need to use the main system preferences dialog:SYSTEM PREFERENCES | GENERAL | PREFER TABS [NEVER | IN FULL SCREEN | ALWAYS] WHEN OPENING DOCUMENTS.When set to ALWAYS then every time you open a new document in all supported applications, then it will open in a new tab. If you

MacVector 11.1.2 Updater

Are using MacVector 15.0.1 or later, you will find that this interface has had some significant tweaks. First, a new CRISPR Indel Detection mode has been added to the Align To Reference settings dialog. This largely removes any need to adjust the individual settings;The second change is that a clean up step has been added to the alignment algorithm to minimize the number of gapped segments in the final alignment. This has the effect of dramatically cleaning up the region around the indels. Compare the alignment below to the previous MacVector 14.5.3 generated alignment;This is an article in a long running series of tips to help you get the most out of MacVector. If you want to get notified every time a new tip gets published, follow us @MacVector on twitter (or check the feed for the hashtag #101MacVectorTips) or like us on Facebook. How to use Codon Preference plots By Chris | Published: August 16, 2016 When you are looking for open reading frames in newly sequenced regions, it’s not always the longest ORFs that are protein-encoding. Lets look at an example from one of the sequences included with MacVector:/Applications/MacVector/Sample Files/Gal Cosmid.nucl.This is from Streptomyces coelicolor, a filamentous bacteria with a 73% G+C content. The high G+C% means that stop codons (TAA, TAG and TGA) occur relatively infrequently by chance, so long open reading frames are quite common. Look at this plot from an Analyze | Nucleic Acid Toolbox plot;You can see there is a long open reading frame in the top most pane in Frame +3. However, this is extremely unlikely to actually encode a functional protein. How do we know? Take a look at the Staden Codon Preference plots in panels 3 and 4. These plot the probability that each of the three frames encodes a protein. Happy Holidays from the MacVector Team; MacVector Pro is now MacVector Pro with Assembler; MacVector 18.7.3: a minor bug fix release; Navigation; Getting Started with MacVector. Getting Started with MacVector: An overview of primer design workflows in MacVector. Getting Started with MacVector: assembling sequence data with MacVector and Happy Holidays from the MacVector Team; MacVector Pro is now MacVector Pro with Assembler; MacVector 18.7.3: a minor bug fix release; Navigation; Getting Started with MacVector. Getting Started with MacVector: An overview of primer design workflows in MacVector. Getting Started with MacVector: assembling sequence data with MacVector and

MacVector 11.0.4 Updater

MacVector 18.5.1 Chris | Published: December 21, 2022 We’ve just released a minor update, MacVector 18.5.1.Changes include:Further enhancements for importing Sequencher projects (.spf) including heavily edited assemblies.A new setting for always opening MacVector in “light” mode even when macOS switches to “dark” mode.Easier remote activation of standard licenses for larger sites.increased number of seats for serverless network licenses.You will be automatically promoted to update. or go to FILE | CHECK FOR UPDATES… to update now.You can download the full installer instead.For assistance with remove activation of standard licenses please contact support. MacVector 18.5 has just been released and is macOS Ventura ready! By Chris | Published: November 11, 2022 It’s that time of year again. Apple have released macOS Ventura and we are very pleased to announce MacVector 18.5 is out. MacVector 18.5 is fully macOS Ventura ready!macOS Ventura readyMacVector 18.5 was developed and tested on macOS Ventura. It is supported on macOS High Sierra to macOS Ventura.MacVector 18.5 is a Universal Binary application and will run natively on Apple Silicon M1 and M2 Macs as well as Intel Macs.Heterozygote Analysis of Sanger trace filesThe heterozygote analysis tool analyzes one or multiple Sanger trace files and reports on all possible heterozygotes.You can also analyze Sanger trace files and permanently change the basecalled sequence with an IUPAC ambiguity code representing the basecalled heterozygote.The tool works on multiple trace files in the Assembly project manager or the Align to Reference editor.You can also basecall heterozygotes in a trace file in the Single Trace Editor.Align to Reference supports long readsLong sequencing reads from PacBio and ONT sequencers can now be assembled in Align to Reference. Miscellaneous enhancementsImporting Sequencher project (.SPF) files has been significantly enhanced.As usual there’s many bug fixes and changes that you probably don’t care too much about! But be assured

MacVector 9.5.2 Installer

One (1587bp) containing most of the reads;So I double-clicked on Contig 1 to open up the contig editor and switched to the Map tab where I could see the restriction sites and an overview of the assembly;Perhaps the entire GalK protein is encoded within the Contig? So I chose Analyze|Open Reading Frames…, accepted the defaults and saw one long ORF running from right to left on the minus strand;After selecting this, its trivial to choose Analyze|Translation… and get the amino acid translation in a separate MacVector window. Finally, lets run a Database|Internet BLAST search on that translated protein to see what we have “cloned”.Surprise! The top hit is a Galactokinase protein from another Clostridium species. So there you have it – sorry about the long post but hopefully this gives you a good feeling for how you can use the MacVector Align To Folder function to clone genes from NGS data sets and validate the results.This is an article in a long running series of tips to help you get the most out of MacVector. If you want to get notified every time a new tip gets published, follow us @MacVector on twitter (or check the feed for the hashtag #101MacVectorTips) or like us on Facebook. Posted in 101 Tips | Download the latest published version of your favorite sequence with its accession number By Chris | Published: September 20, 2016 It’s very quick to download the latest version of a sequence if you know its accession number. When you start working with a new sequence, it’s the best place to start.Go to DATABASE > ENTREZEnter the accession number of your favorite sequenceClick SEARCHDouble click on the result to open up your sequence directly in MacVector.If you do not know the accession number, then it’s still easy, but you might. Happy Holidays from the MacVector Team; MacVector Pro is now MacVector Pro with Assembler; MacVector 18.7.3: a minor bug fix release; Navigation; Getting Started with MacVector. Getting Started with MacVector: An overview of primer design workflows in MacVector. Getting Started with MacVector: assembling sequence data with MacVector and Happy Holidays from the MacVector Team; MacVector Pro is now MacVector Pro with Assembler; MacVector 18.7.3: a minor bug fix release; Navigation; Getting Started with MacVector. Getting Started with MacVector: An overview of primer design workflows in MacVector. Getting Started with MacVector: assembling sequence data with MacVector and

MacVector 10.5.3 Updater

Chris | Published: March 3, 2017 When you run a Blast search, as well as a list of hits, you will get a list of alignments between your query sequence and each hit. As with most other text alignments in MacVector, identical matches are by default represented by a vertical line (a score greater than 1) and mismatches (whether similar or not) are represented with a space.However, sometimes you are more interested in identifying gaps or mismatches in the Blast hits. For example when you are looking for mismatches in motifs or other protein domains or looking for SNPs in DNA sequences.Most results and displays in MacVector are customizable, and BLAST alignments are no exception. You can also change the length of each line. To change the match characters:Open OPTIONS | ALIGNED SEQUENCEIn the LINES panel change the SCORELINE match characters.The default is to display a vertical line for a hit, and a space for mismatches. In the screenshot below we have changed matches to a space, a “-“ for scores between 1 and -1 and “|” for mismatches with a score less than -1. This makes mismatches very noticeable whilst scrolling through the aligned sequence results.All these changes will be the new defaults until you reset them.To change the line lengthOpen OPTIONS | TEXT VIEWIn the APPEARANCE panel change the LINE LENGTH to the length you prefer.Remember that due to changes at the NCBI, BLAST and Entrez will only work in MacVector 15.1 and later. This entry was posted in Techniques, Tips and tagged weeklytip. Bookmark the permalink. Both comments and trackbacks are currently closed.